DEVELOPMENT OF AN AUTOMATED IMMUNOTURBIDIMETRIC FERRITIN ASSAY PDF

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Such assays are suitable for the measurement of proteins at concentrations >5– 10 mg/L. .. Development of an automated immunoturbidimetric ferritin assay. May 3, Different laboratory methods are used to quantify ferritin . assays and automated equipment developments, and in routine laboratory assays and Seco M. Development of an automated immunoturbidimetric ferritin assay. J. Colloid. Interface Sci. (2): – 3. Borque, L., Rus, A., Bellod, L., and Seco, M.L. Development of an automated immunoturbidimetric ferritin assay.

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The maximum adsorption plateau is achieved at a polyclonal antibody concentrations of 3. The turbidimetric method affords a measuring range of 0. Principle of Test Method: Clin Chem ; 42 Suppl 6: Production of C-reactive protein and risk of coronary events in stable and unstable angina. Among the many commercially available assays for CRP, the most common use fluid-phase or particle-enhanced turbidimetric or nephelometric procedures 7. Colloidal stability and immunoreactivity.

Covalent isotherm A and dose—response curves as a function of antibody type and coverage B and particle size C. It allows lower antibody coverage on the microparticle reagent and provides highly sensitive and robust particle-enhanced turbidimetric immunoassay for serum CRP. In conclusion, a low coverage 0.

It is also used to distinguish between hypoferric anemia and hypochromic anemia chronic infection and tumor anemias, sideroblastic anemia or thalassemia.

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Development of an automated immunoturbidimetric ferritin assay.

Clin Chem ; 45 Suppl 6: Show more Technical Briefs. Eur J Pediatr ; Evaluation of an improved immunonephelometric assay for Developmetn protein [Abstract].

Ferritin is an acute-phase respondent, and so individuals with conditions association with an inflammatory response may have elevated serum ferritin levels that are not directly related iron stores. CRP measurements have been used for many years in the management of a variety of clinical situations, such as bacterial infections, ischemic necrosis of tissue, and active inflammatory conditions 1. Clinical Chemistry Nov46 11.

We found that at any given working dilution of immunoturbidimetrid studied, the initial absorbance decreases with increasing wavelength and increases with microparticle diameter, according to the light-scattering theory.

Clin Chem ; A microparticle size of nm and a wavelength of nm were selected as the best choice because of the high slope and wide dynamic range showed. We monitored the reaction kinetics at pH values of 6. For practical use, it is essential to obtain microparticle reagents colloidally stable under reaction and storage conditions, with the stability often depending on protein coverage.

Development of an automated immunoturbidimetric ferritin assay.

Download figure Open in new tab. Diagnostic use of serum ferritin encompasses prelatent and latent iron deficiency as well as iron overloading. Recent reports show median values in healthy controls of aasay.

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Posttreatment with additives BSA, surfactants has been proposed 13 14although it reduces IgG charge and reactivity. The ferritin assay is an automated immunoturbidimetric method. The microparticle reagents were prepared according to a previously published method by the carbodiimide [1-ethyl 3-dimethylamino-propyl carbodiimide chloride] procedure The microparticle reagent response of the different antibodies to increasing CRP concentrations can be seen in Fig.

C-reactive protein and bacterial infection in preterm infants. We then selected 0. Research Article Technical Brief. Ferritin determinations are widely used for monitoring renal anemia when iron utilization and distribution disorders are present during therapy with erythropoietin. J Biomater Sci Polym Ed ; 3: We describe a microparticle reagent that we use in an optimized turbidimetric procedure with immunopurified polyclonal antibodies against CRP.

Some use expensive monoclonal antibodies, and others use higher sample volumes and antibody concentrations in the reaction mixtures, which can increase nonspecific reactions We have developed a new procedure for turbidimetric measurement of ferritin concentration in human serum, based on latex microparticle agglutination technology.

Lancet ; Biochemical markers and hematologic indices in the diagnosis of functional iron deficiency.