BM and the effect on DNA cleavage induced by H2O2 UV-photolysis was investigated. cytotoxicity and DNA damage in human non-immortalized fibroblasts. from CP, PK, WS and the effect on DNA cleavage induced by H2O2 UV- photholysis. cytotoxicity and DNA damage in human non-immortalized fibroblasts. methanol extract of BM and the effect on DNA cleavage induced by H2O2 UV- photolysis cytotoxicity and DNA damage in human non-immortalized fibroblasts.

Author: Gushura Shakak
Country: South Africa
Language: English (Spanish)
Genre: Politics
Published (Last): 18 November 2014
Pages: 231
PDF File Size: 17.70 Mb
ePub File Size: 2.63 Mb
ISBN: 311-4-22936-479-4
Downloads: 2415
Price: Free* [*Free Regsitration Required]
Uploader: Voodoosho

Metabolic alterations in lung cancer-associated fibroblasts correlated with increased glycolytic metabolism of the tumor. Our work demonstrate that irradiated CAFs lose their pro-tumorigenic potential in vivo, affecting angiogenesis and tumor engraftment.

These studies support the hypotheses that cancer cells increase glucose metabolism to compensate for excess metabolic production of ROS and that inhibition of glucose and hydroperoxide metabolism may provide a biochemical target for selectively enhancing cytotoxicity and oxidative stress in human cancer cells.

The plants exhibit varying degrees of therapeutic value some of which useful in the treatment of cognitive dysfunction, epilepsy, insomnia, rheumatism, gout, dyspepsia. CAFs and NFs were isolated from fresh specimens of colorectal cancer and their paired normal colon tissue and cultured by tissue explant method. Although cancer-associated fibroblasts CAFs are viewed as a promising therapeutic target, the design of rational therapy has been hampered by two key obstacles.

Using a miRNA expression profiling array, we determined the miRNA expression profile of secretory exosomes in CRC cells and then identified potential miRNAs with significant differential expression compared to normal cells via enrichment analysis. Using this procedure data from each experiment were normalized to the corresponding normal cell type and combined for analysis [ 1920 ].


Free radical scavenging capacity and protective effect of Bacopa monniera L. on DNA damage.

Flow cytometry was used to quantify the proportion of apoptotic cells. The tumor microenvironment orchestrates the sustained nonimmortalizzed, metastasis and recurrence of cancer. FAP positive fibroblasts induce immune checkpoint blockade resistance in colorectal cancer via promoting immunosuppression.

Solid tumors possess a unique microenvironment characterized by local hypoxia, which induces gene expression changes and biological features leading to poor outcomes. The results with antimycin A also support the hypothesis that tumor cell mitochondria have a greater capacity for producing superoxide, relative to normal epithelial cell mitochondria.

Brain metastases fibroblastw associated with high morbidity as well as with poor prognosis and survival in breast cancer patients.

Indian medicinal plants as antiradicals and DNA cleavage protectors.

Redox gene therapy protects human IB-3 lung epithelial cells against ionizing radiation-induced apoptosis. Let-7b inhibits cancer-promoting effects of breast cancer-associated fibroblasts through IL-8 repression. We prioritized several potential pan-cancer therapeutic targets that are likely to have high specificity for activated CAFs and minimal toxicity in normal tissues.

Both the adhesion and spreading were proposed to be mediated by GPER, since G1 also stimulated these effects similar to E2, and G15 reduced them. It has been reported that stromal cell features may affect the clinical outcome of breast cancer patients.

Activation of lipid metabolism is an early event in carcinogenesis and a central hallmark of many tumors. These findings support a critical role for calcium signaling during CAF differentiation and highlight a novel, repurposable modality for cancer therapy.

However, the mechanism by which CAFs develop in the tumor microenvironment remains unknown. Colorectal CICs were resistant to conventional chemotherapy in cell-autonomous assays, cytofoxicity CIC chemoresistance was also increased by cancer-associated fibroblasts CAFs. Importantly, we showed that oxidatively transformed FIBs isolated from composite tumor xenografts retained their ability to fibroblast tumor growth and aggressiveness when adoptively transferred into new xenografts.


High stromal expression levels of FAP correlate with poor prognosis. Vicent, Silvestre; Sayles, Leanne C.

Control Panel – Login

There is increasing evidence that cancer-associated stroma plays a role in both nonimmoralized progression and carcinogenesis. Cancer cells, relative to normal cells, demonstrate increased sensitivity to glucose deprivation-induced cytotoxicity. The aim of this study was to identify the anti-cancer mechanism of Polyphyllin I PPI on gastric cancer cells via its activity on cancer-associated fibroblasts CAFs.

Abstract Cancer cells, relative to normal cells, demonstrate increased sensitivity to glucose deprivation-induced cytotoxicity.

These 2, proteins were analyzed in the Human Protein Atlas online database by comparing their immunohistochemical expression patterns in normal versus tumor-associated fibroblasts. CAFs show differences with NFs in morphology, characteristics of activation and secretion of some cytokines.

There was a problem providing the content you requested

Specific inhibition of fibroblast activation protein FAP -alpha prevents tumor progression in vitro. In all, 29 laryngeal squamous cell carcinoma specimens were collected and primarily cultured.

These results strongly suggest that depletion of NADPH levels in colon and breast carcinoma cells could contribute to glucose deprivation-induced cytotoxicity and oxidative stress in cancer cells.

Exosomes from CRC cells contained significantly higher levels of miRb than did exosomes from normal colorectal epithelial cells.